Analysis of Organic Acids with Ultisil OAA Column
What is organic acid?
Organic acids refer to organic compounds with acidic properties. The most common ones are associated with functional groups of carboxyl (-COOH), sulfonic group (-SO3H), sulfinic group (-SO2H), such as malic acid, tartaric acid, oxalic acid, formic acid, etc. Organic acids have wide applications in oil and gas well stimulation treatments, food preservation, nutrition, animal feed and biological system.
Reversed phase high performance liquid chromatography (HPLC) is the most widely used method for the separation and analysis of organic acids. Usually, acidic aqueous mobile phase is used to prevent the dissociation of organic acids and improve the retention in the hydrophobic column. This improves the separation efficiency for various organic acids. However, even under acidic conditions, the polarity of those organic acids with strong acid functional groups is still high and difficult to retain on C18 column. In addition, the high aqueous mobile phase can cause the phase collapse in conventional C18 column and thus deteriorate the separation performance and lifetime of the column.
- Excellent separation capability of hydrophilic organic acids.
- Compatible with 100% aqueous mobile phase, good retention for polar compounds.
- Ideal selectivity for a variety of organic acids, with high separation efficiency and excellent peak shape.
- Each column is tested to ensure excellent hydrolysis stability for hydrophilic organic acids analysis.
- Excellent in separating hydroxyl fatty acids and aromatic organic acids, best choice for organic acids analysis in the pharmaceutical industry, food, and beverage detection industry.
| 1. Oxalic acid | 5. Pyruvic acid | 9. Acetic acid | 13. Cis-aconitic acid |
| 2. Tartaric acid | 6. Malic Acid | 10. maleic acid | 14. Acrylic acid |
| 3. Quinic acid | 7. Lactic acid | 11. Citric acid | 15. Propionic acid |
| 4. Methanoic acid | 8. Ascorbic acid | 12. Fumaric acid | 16. Citraconic acid |
- HPLC column：Welch Ultisil® OAA，4.6×300mm
- Mobile phase：A：0.01mol/L KH2PO4 solution, B：methanol, gradient elution
- Temperature：32℃
- Flow Rate：0.5 mL/min
- Detector：UV 210nm
- Injection Volume：5μL
For product inquiry or any other help, please send email to sales@auroraprosci.com.
Related Articles
Analysis of Amino Acids with Ultisil Amino Acids Plus HPLC Column
Amino acids are the basic constituents of proteins and also intermediates in metabolic pathways. Analysis of amino acids is high demanded in the pharmaceutical research and food industry for protein characterization, cell culture monitoring, and nutritional analysis of foods and feeds. Reverse phase HPLC was widely used for the separation of the amino acids. However, direct HPLC analysis of amino acids has some challenges：
- Most of amino acids are very polar due to their carboxyl group(-COOH) and amino group (NH2). This results in poor retention in Conventional reversed-phase conditions.
- Most of the amino acids and their substituent groups have poor UV absorbance and poor sensitivity in detection.
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Tags Organic acids, Pharmaceutical, Food industry, HPLC, Separation science