How to Calibrate the Flow Rate of a Peristaltic Metering Pump?

For a specific ID  tubing with a certain length, the flow rate of peristaltic pump is proportional to the pump drive speed (RPM) within the linear region of its operation.  The actual flow of a peristaltic pump depends on many factors, such as the fluidics properties (viscosity), the way the tubing installation, system hydraulic resistance (fitting, tubing flow resistance), the tubing stiffness and operation time as well fluid temperature.  These factors cannot be accounted during factory testing and calibration.   The flow rate even changes a little bit after tubing burn-in (first 1-2 hours operation).   One can easily notice that the flow rate of a peristaltic pump gets attenuated.  Calibrating a peristaltic pump becomes necessarily to ensure accurate dispensing of liquids.  The steps can vary based on the specific model and features of your peristaltic pump, but here are general guidelines for calibrating a peristaltic pump with a calibration function. 


Test Equipment and Materials: 

# Lab graduated cylinder

# Precision balance for gravimetric analysis 

# Testing fluid: DI water

# Longer Pump Laboratory Peristaltic Pump L100-1S 

# Longer Pump Peristaltic Pump Head YZ1515X-A (3 rollers)

# Stop watch for recording the dispensing duration

# Pump tubing – Tubing Sizes #15 for example


Test Procedure and Method: 

The primary method of a peristaltic pump flow rate calibration is to measure the total amount of liquid dispensed within a certain time duration.  This measurement can be done by either directly measuring the delivered volume or the weight (gravimetric method).  Then, the mass of the dispensed liquid can be converted into volumetric flow rate by dividing the density of the liquid.  The flow rate can be expressed as follows: 

  • Mass flow rate = weight (gram)/ time duration (g/min)  OR 
  • Volumetric flow rate = volume (mL)/ time duration (mL/min) 


The following describes the basic steps to calibrate a peristaltic pump: 

  1. Read the Manual: Familiarize yourself with the user manual provided by the manufacturer. It will contain specific instructions for your pump model.
  2. Gather Calibration Equipment: Ensure you have the necessary equipment for calibration, such as a measuring container, stopwatch, and any calibration solutions specified by the manufacturer.
  3. Prime the Pump: Prime the pump to remove any air bubbles from the tubing. This ensures accurate calibration.
  4. Set Up the Calibration Environment: Set up the pump in the environment where it will be used. Factors such as temperature and humidity can affect calibration.
  5. Access Calibration Mode: If your peristaltic pump has a dedicated calibration mode, access it according to the instructions in the manual. Some pumps may have a calibration button or menu option.
  6. Enter Calibration Parameters: Input the necessary calibration parameters, such as the desired flow rate, volume, or time. Follow the prompts provided by the calibration function.
  7. Start Calibration: Initiate the calibration process. The pump may run through a series of dispensing cycles at the specified parameters.
  8. Measure and Adjust: Use the measuring container and stopwatch to record the actual dispensing volume or time. Compare this to the expected values entered during calibration. If there is a discrepancy, adjust the calibration settings accordingly. Some pumps allow you to manually adjust factors like flow rate or dispensing time.
  9. Repeat if Necessary: If the initial calibration is not within the desired accuracy, repeat the process until satisfactory results are achieved.
  10. Save Calibration Settings: If your pump has the capability to save calibration settings, make sure to save the adjusted parameters to memory.
  11. Perform Validation Tests: After calibration, perform validation tests by dispensing known volumes or running the pump for a specified time to ensure accuracy.
  12. Document Calibration: Document the calibration process, including the adjusted settings and any deviations from the expected values. This documentation can be useful for future reference and quality control.

Always follow the manufacturer's guidelines and recommendations for calibration specific to your peristaltic pump model. Calibration procedures may vary, and it's important to adhere to the provided instructions for accurate and reliable pump performance.  The calibration demonstration video of Longer Pump L100-1S Peristaltic Pump is for reference only. 


If you have questions or need assistance on calibrating a peristaltic pump, please feel free to reach out to us. 



  • How to Calibrate the Flow Rate of a Peristaltic Metering Pump?

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Tags Sample Preparation, Drug Delivery, Cell Culture, Metering Pump, Peristaltic Pump