The Gradient PCR Optima 96 features six separate thermo-electric modules for precise temperature control and gradient PCR to ensure performance on the edges of 96 well plates. The device is easy to use with a high resolution, 7-inch LCD touchscreen panel, and has many advanced features such as touchdown (TD) PCR mode and long-range PCR modes. The Optima 96 also boasts one of the fastest heating/cooling rates for better nucleotide pairing performance. Designed to be compatible with PCR tubes, PCR 8 strips, and 96-well plates, this device is an excellent choice for researchers seeking precise temperature control.

Key Features

Capable to run 96 samples at once with 6 individual heating blocks to provide precise temperature control and PCR optimization
Accurate and sensitive: 6 separate thermoelectric modules resulting in high level of accuracy and sensitivity in detecting and amplifying target DNA sequences, even in low concentrations
Quicker heating and cooling: equipped with one of the fasting heating and cooling rates in industry with a max heating rate of >5°C/s nd max cooling rate of >4°C/s. This feature ensures better nucleotide pairing performance and reduces the overall time required for PCR reactions.
Versatile components: Segmented heating lid design automatically adjusts to different types of PCR tubes, strips and plates, making it compatible with a wide range of PCR applications. The device offers advanced features such as touchdown (TD) PCR mode and long-range PCR modes, further enhancing versatility and customization of PCR process.
Accurate results and easier data storage: 6 separate imported thermoelectric modules ensures accurate results for PCR optimization and performance on the edges of 96 well plates. Including a front-facing USB interface for easy program import and export, software updates and multiple accounts and access rights.
Save user profiles and experiment for future use and accommodate different types of PCR tubes, strips, and plates (96-well plates, 12x PCR strips, 96
Two years all inclusive warranty

Typical Applications

Cell culture in drug delivery, vaccine development and process development optimization
Gene expression analysis, DNA sequencing, mutation detection, and cloning

Product Model	SK40/ PCR100202
Sample Capacity	96-well plates 12x PCR 8 strips 96x0.2 mL well plates
PCR reaction volume	10-100 µl
Heating lid temperature	30-110°C
Max. block heating rate	5°C/s
Max. block cooling rate	4°C/s
Sample temperature range	4°C-100°C
Temperature uniformity	<0.5°C (20s after reaching 95°C )
Temperature accuracy	±0.1°C (35 °C - 99.9°C)
Technology(heating/cooling)	Semiconductor Peltier technology 6 independent blocks
Display resolution	0.1°C
Maximum temperature difference between blocks	5°C between two adjacent blocks
Difference between two adjacent blocks	0.1°C-5.0°C
Gradient temperature accuracy	9±0.1°C (35°C - 99.9°C)
Gradient temperature accuracy	<0.5°C (20s after reaching 95°C)
Display	7" LCD touch screen real-time display of PCR process
Time increment/decrement	1-120s for long Range PCR experiment
Temperature increment/decrement	0.1°C-10.0°C for Touchdown PCR
Maximum number of cycles	Unlimited

Workflow

Prepare and load PCR reaction mixture

Include template DNA, primers, nucleotides, and Taq polymerase and load reaction mixture into PCR tube. Select a prebuild program, modfiy program template or build a new thermocycling program from scratch

Set annealing temperature range

Optima 96 has six independent heating/cooling modules for PRC optimization and excellent gradient temperature accuracy (+0.1°C). A 5 °C differential can be set between each of the 6 modules allowing for ultimate PCR optimization and 25 °C span for analyzing performance temperatures in a single run.

Start and monitor PCR reaction

Monitor results displayed on a Optima 96's new 7-inchLCD touchscreen panel, making it easy to setup and analyze for performance. Quickly modify and save programs and watch your gels come to life. Designed to be used in GMP and non-GMP PD laboratories or for routine PCT workflows.