FluoSurf-O™ is a high-performance fluorinated surfactant designed and optimized to stabilize aqueous droplets in fluorinated oils for chemical or biotechnological applications. FluoSurf-O™ is an inert block copolymer designed to stabilize droplets containing biological compounds. It is suitable for droplet-based microfluidic experiment such as droplet digital polymerase chain reaction (ddPCR) or single cell analysis. Thanks to its low auto-fluorescence, FluoSurf-O™ is particularly efficient for fluorescent dyes detection even at low concentration.

Key Features:

Stability: FluoSurf-O™ allows the stabilization of droplets from 1 to 300μm with a high generation frequency and keeps droplets stable during heating cycles.
Biocompatibility: FluoSurf-O™ is biocompatible and can be used to stabilize droplets containing biochemical compounds or biological entities.
Purity: FluoSurf-O™ is obtained with a high purity.
Leakage control: Hydrophilic and hydrophobic molecules can be efficiently contained within droplets
Excellent reproducibility

Typical Applications

Encapsulating cells, proteins, DNAs, and RNAs
High-throughput screening of conditions for protein crystallization
Single-cell high-throughput genome sequencing
Single-cell high-throughput screening
Single cell analysis of protein
Quantitative detection of single-cell protein expression
Barcoding for single-cell transcriptomics in microdroplets
Single cell enzymatic kinetics

Product Part Number	FluoSurf-O™
Solvents	Fluorinated oils such as Fluo-Oil 7500, Fluo-Oil 40, Fluo-oil 135 and Fluo-Oil 200
Formula	PFPE-b-PPO-PEO-PPO-b-PFPE
Molecular weight	7kDa<Mw<13kDa
Charge	Neutral
Interfacial Tension at 4wt% in HFE 7500	10 mN/m
CMC in HFE 7500	0.2 w/w%
Hazards	Not classified hazardous. SDS available
Biocompatibility	Has been tested with plankton, yeast, E.coli and mammalian cells