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High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used to separate, identify, and quantify components in a mixture. It operates on the principle of chromatography, where the sample is dissolved in a solvent (mobile phase) and passed through a column packed with a stationary phase. The components of the sample interact differently with the stationary phase, causing them to separate based on their respective chemical properties, such as size, polarity, and affinity.
Microfluidics, a rapidly evolving field at the intersection of physics, engineering, chemistry, biology, and biotechnology, has revolutionized numerous aspects of life science, biotechnology, and biopharmaceutical industries. With its ability to manipulate small volumes of fluids on the microscale, microfluidics offers unprecedented control and precision, leading to a wide array of applications. Here's an overview of microfluidics applications in these fields and some representative products showcasing the transformative impact of microfluidic technologies on research, diagnostics, and therapy development.
One of the key advantages of peristaltic pumps is their ability to handle delicate and viscous fluids without damaging them. Unlike other types of pumps, the flexible tubing used in peristaltic pumps does not come into contact with the fluid, which means that there is no risk of the fluid being damaged or contaminated. Peristaltic pumps are also known for their reliability and durability. They are simple in design and have few moving parts, which means that they require minimal maintenance and have a long lifespan. They are also able to operate in a wide range of temperatures and environments, making them suitable for use in a variety of industries.
The Vacuum Manifolds have been specifically designed to enhance the efficiency of high-throughput processes such as nucleic acid purification, solid phase extraction, protein precipitation, Oligo synthesis, and various other applications. These manifolds are tailored to accommodate 48/96/384 well plates and Luer-inlet columns, streamlining procedures by eliminating the need for repetitive pipetting and centrifugation inherent in traditional nucleic acid extraction methods.
A centrifuge operates on the principle of centrifugal force, which is the outward force experienced by an object rotating around a central point. In the context of a centrifuge, this force is used to separate components of a sample based on their density. The basic components of a centrifuge include: Rotor: The rotor is the spinning component of the centrifuge where sample tubes or containers are placed. It rotates at high speeds, creating the centrifugal force necessary for separation. Motor: The motor powers the rotation of the rotor, generating the centrifugal force that acts on the samples. Control System: The control system allows users to set parameters such as speed, time, and temperature. Programmable features enable customization for different types of separations.
For a specific ID tubing with a certain length, the flow rate of peristaltic pump is proportional to the pump drive speed (RPM) within the linear region of its operation. The actual flow of a peristaltic pump depends on many factors, such as the fluidics properties (viscosity), the way the tubing installation, system hydraulic resistance (fitting, tubing flow resistance), the tubing stiffness and operation time as well fluid temperature. These factors cannot be accounted during factory testing and calibration. The flow rate even changes a little bit after tubing burn-in (first 1-2 hours operation). One can easily notice that the flow rate of a peristaltic pump gets attenuated. Calibrating a peristaltic pump becomes necessarily to ensure accurate dispensing of liquids. The steps can vary based on the specific model and features of your peristaltic pump, but here are general guidelines for calibrating a peristaltic pump with a calibration function.
Perfect vacuum or 100% vacuum is a zero reference pressure. In practice, a perfect vacuum is impossible to obtain. The two tables below are for reference only when selecting vacuum pump related products. It is the sole responsibility of users who uses these tables to select their products. Any improper selection or use of the table may cause any damage or injury. Please consult with your own subject matter expert for advice on how to select appropriate vacuum. Vacuum Pressure Unit Conversion Chart (Absolute) and Vacuum Pressure Unit Conversion Chart (Gauge) are plotted for reference.
Preventive maintenance is crucial to extend the life of peristaltic pump tubing and ensure optimal performance. Here are some general steps you can take to maintain peristaltic pump tubing: Regular inspection, Replace tubing regularly, Proper tubing material selection, Clean the pump head, flush the tubing, Avoid dry running, Maintain proper tubing compression, Monitor pump performance, Train operators, Documentation and Environmental considerations.
Vacuum filtration is a critical technique applied in laboratories and industrial processes for the separation of solids from liquids through the use of reduced pressure. Widely utilized in fields such as chemistry, biology, the pharmaceutical industry, and environmental analysis, this method efficiently removes particles, clarifies solutions, and isolates precipitates or solid residues. Also known as negative pressure filtration, its fundamental principle involves creating negative pressure beneath the membrane, leveraging the pressure differential to accelerate liquid flow and achieve effective solid-liquid separation. A typical vacuum filtration setup includes the following components.
The vacuum pump in a spin coater is typically used to remove air from the space between the substrate and the chuck, ensuring good contact and adhesion between the substrate and the coating material. The vacuum helps to eliminate air pockets and enhance the uniformity of the coating. Here's a general guide on how to use the vacuum pump in a spin coater and the practical tips and considerations.